Vitamin C indicator solutions?

For my science project I need to see which form of Orange juice obtains the largest amount of vitamin C.





What is a vitamin C indicator solution?


Where can I get it?


How do I use it?


Is there a (color)chart of some sort that can possibly tell me how much vitamin C is in each form of O.J.?

Vitamin C indicator solutions?
Dichlorphenolindophenol


DCPIP is commonly used as an indicator for Vitamin C. If vitamin C, which is a good reducing agent is present , the blue dye, which turns pink in acid conditions and is reduced to a colourless compound by ascorbic acid.





DCPIP (blue) + H+ ----------%26gt; DCPIPH (pink)


DCPIPH (pink) + VitC ----------%26gt; DCPIPH2 (colourless)


C6H8O6 + C12H7NCl2O2 ----------%26gt; C6H6O6 + C12H9NCl2O2


In a titration, when all the ascorbic acid in the solution has been used up, there will not be any electrons available to reduce the DCPIPH and the solution will remain pink due to the DCPIPH. The end point is a pink colour that persists for 10 seconds or more.
Reply:i am doing the same science project this year the vitamin c indicator solution recipe can be found on http://www.accessexcellence.org/AE/ATG/d...





once u have made it, u will take 15 mL (per test) and then, using a pipette (liquid eyedropper), drop the orange juice being tested and record the amt. of drops used to turn the indicator clear. the directions to the experiment can be found





http://www.accessexcellence.org/AE/ATG/d...